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EBI-ENA scheduled disruption, no submissions 12-16 May 2025.

Oxalic acid treatment of honey bee hive - ID 15168

Alias: OABEEHIVE
Abstract: Study of the effect of 3 oxalic acid treatments (tricking at 2.1%, tricking at 4.2%, and sublimation through Varrox®) upon the microbial communities (bacterial and eukaryotic) associated with workers’ gut, hive bee bread and pupae, sampled from 3 apiaries. Apiaries were managed through conventional or ecological beekeeping practices, and were under different anthropization levels (located in an urban, rural or mountainous area). Hives were sampled pre- and post-treatment by doing: i) queen caging and pre-treatment sampling on August 2017, ii) queen liberation and hive treatment 24 days later, and iii) post-treatment sampling approximately 20 days later (middle of October 2017). Characterization was performed via amplification of the V4 region of the 16S rRNA gene for bacteria, and the ITS2 region for eukaryote.
Description: Characterization of the effect that 3 different oxalic acid treatments (tricking at 2.1%, tricking at 4.2%, and sublimation through Varrox) have on the bacterial and eukaryotic communities of honey bee worker's gut, black-eyed pupae and bee bread.
Owner: june.gorrochategui@ehu.eus
Publications:
PI: Iratxe Zarraonaindia (UPV/EHU)
EBI: ERP151279 (submitted)
Artifacts
ID Data Type Platform Target Gene Target Subfragment Algorithm
179399     16S Illumina 16S rRNA V4 Trimming (length: 200)
181710     16S Illumina 16S rRNA V4 Trimming (length: 90)
181711     16S Illumina 16S rRNA V4 Trimming (length: 100)
181712     16S Illumina 16S rRNA V4 Trimming (length: 150)
179499     ITS Illumina ITS ITS2 Trimming (length: 200)
179402     16S Illumina 16S rRNA V4 Pick closed-reference OTUs (reference-seq: /databases/gg/13_8/rep_set/97_otus.fasta) | Trimming (length: 200)
181717     16S Illumina 16S rRNA V4 Pick closed-reference OTUs (reference-seq: /databases/gg/13_8/rep_set/97_otus.fasta) | Trimming (length: 90)
181719     16S Illumina 16S rRNA V4 Pick closed-reference OTUs (reference-seq: /databases/gg/13_8/rep_set/97_otus.fasta) | Trimming (length: 100)
181720     16S Illumina 16S rRNA V4 Pick closed-reference OTUs (reference-seq: /databases/gg/13_8/rep_set/97_otus.fasta) | Trimming (length: 150)
179504     ITS Illumina ITS ITS2 Pick closed-reference OTUs (reference-seq: /databases/unite/8.0/sh_refs_qiime_ver8_97_s_all_02.02.2019.fasta) | Trimming (length: 200)
179398     16S Illumina 16S rRNA V4 Split libraries FASTQ
179498     ITS Illumina ITS ITS2 Split libraries FASTQ
179400     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: all.biom) | Trimming (length: 200)
179401     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: reference-hit.biom) | Trimming (length: 200)
181721     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: reference-hit.biom) | Trimming (length: 100)
181722     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: all.biom) | Trimming (length: 90)
181723     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: all.biom) | Trimming (length: 100)
181724     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: reference-hit.biom) | Trimming (length: 90)
181731     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: all.biom) | Trimming (length: 150)
181734     16S Illumina 16S rRNA V4 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: reference-hit.biom) | Trimming (length: 150)
179502     ITS Illumina ITS ITS2 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: all.biom) | Trimming (length: 200)
179503     ITS Illumina ITS ITS2 Deblur 2021.09 (Reference phylogeny for SEPP: Greengenes_13.8, BIOM: reference-hit.biom) | Trimming (length: 200)
179182     16S Illumina 16S rRNA V4
179497     ITS Illumina ITS ITS2