This workflow will (1) filter reads mapping to rRNA genes, and (2) run resulting sequences against WoLr2 with Woltka to generate per-genome and per-gene feature-tables at various taxonomic and functional levels.
This workflow relies on SortMeRNA, and Woltka.
More infoNote that the current recommendation is to run Woltka with the two available databases: RS210 and WoLr2 as they give different perspectives from the same data. Additionally, this workflow starts from the rawest data available.
Note that, for human host samples, human sequence removal must be completed independently BEFORE upload to comply with Qiita’s
Terms of Use.